1. Upload File: Begin by uploading an input file with the three essential columns: Gene Name Log2 Fold Change (log2FC) p-value Optionally, users may provide an additional column containing genes of interest or choose genes directly from a dynamically generated dropdown list that is populated using the input file.
2. Select Columns: After uploading the file, users will need to specify which columns correspond to the necessary data types. This is done by selecting the columns from the dropdown list, which is dynamically generated based on the content of the uploaded file. Choose the appropriate columns for:
- Gene Name
- Log2 Fold Change (log2FC)
- p-value
Once the selections are made, click the "Confirm" button.
3. Generate Volcano Plot: The Volcano Plotter will generate a volcano plot with default parameters. Users can review the initial plot and then proceed to customize it according to their preferences.
4. Customization Options: The user has the option to further customize the volcano plot. More customizable options are available in the section Advance. These options allow users to modify the appearance and styling of the plot to meet their specific requirements.
5. Retrieve Plots: Once the desired customization is applied, users can retrieve the generated plots in their preferred DPI (dots per inch) and format. This ensures that the plots meet the specific needs of the user.
6. Theme Options: Users can choose to apply available themes, upload custom themes, or export the current theme configuration for future use. This offers a high degree of flexibility in visual presentation.
7. Examples: Here are three examples to test and run.
For more information please visit the Help Page
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Read the Instructions to understand its working
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Note :: Results will automatically be removed from the servers after 30 days