Information
Calcium regulates many biological systems and physiological functions by interacting with proteins with different affinities within the cell and in the extracellular matrix. Improper calcium-binding affinity of calcium-binding proteins is one of the major causes of many human diseases related to the overloading or depleting cellular calcium concentration. In calcium binding proteins there is an increase in stability and changes in conformation with the binding of calcium ion. Calcium-binding proteins have been shown to exhibit from μM to mM range affinities depending upon the environment.
Identification of canonical EF-hand loops
Estimation of binding affinity
Using the Webserver for Identification Of EF-hand Calcium Binding Loop and to get an estimate of Binding affinity